Degradation of Cowpea Chlorotic Mottle Virus Ribonucleic Acid in situ
نویسندگان
چکیده
منابع مشابه
An Improved Method for the Preparation of the Four Ribonucleic Acids of Cowpea Chlorotic Mottle Virus
The four components of the genome of cowpea chlorotic mottle virus have been prepared in a highly active and highly purified state by a method based on their resolution by polyacrylamide gel electrophoresis. Activity and purity have been confirmed by gel electrophoresis under denaturing and non-denaturing conditions, infectivity tests on whole plants, translation in an mRNA dependent rabbit ret...
متن کاملA method for the purification of large quantities of biologically active ribonucleic acid components from cowpea chlorotic mottle virus, a multicomponent plant virus.
Cowpea chlorotic mottle virus RNA has been prepared in comparatively high yield (at least 50%) by a modified phenol extraction method. The preparation, which has high biological activity, has been resolved into four components by zonal centrifugation on a 15-40% (w/v) sucrose density gradient. The components obtained have been tested for biological activity against whole plants and plant protop...
متن کاملThe structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus.
The structure of cucumber mosaic virus (CMV; strain Fny) has been determined to a 3.2-A resolution using X-ray crystallography. Despite the fact that CMV has only 19% capsid protein sequence identity (34% similarity) to cowpea chlorotic mottle virus (CCMV), the core structures of these two members of the Bromoviridae family are highly homologous. As suggested by a previous low-resolution struct...
متن کاملStructural rigidity in the capsid assembly of cowpea chlorotic mottle virus
The cowpea chlorotic mottle virus (CCMV) has a protein cage, or capsid, which encloses its genetic material. The structure of the capsid consists of 180 copies of a single protein that self-assemble inside a cell to form a complete capsid with icosahedral symmetry. The icosahedral surface can be naturally divided into pentagonal and hexagonal faces, and the formation of either of these faces ha...
متن کاملReplication and Properties of Cowpea Chlorotic Mottle Virus in Resistant Cowpeas
WYATT, S. D., and C. W. KUHN. 1979. Replication and properties of cowpea chlorotic mottle virus in resistant cowpeas. Phytopathology 69:125129. Although cowpea chlorotic mottle virus (CCMV) caused no symptoms pool of RNA formed soon after inoculation. Several properties of CCMV on cowpea P1 186465, virus could be isolated from the inoculated primary produced in the resistant plants were similar...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1978
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-39-1-131